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	Provedor de dados BJMBR (3) Autor Pinheiro,N.A. (3) Villa,L.L. (3) Andrade,J.A.D. (1) Cerutti,J.M. (1) Dalla Torre,C.A. (1) Maciel,R.M.B. (1) Monteiro,E. (1) Moura,R.P. (1) de Toledo,S.R.C. (1) Mais... Palavra-chave PCR (2) SSCP (2) Silver staining (2) Cancer (1) DNA sequencing (1) HPV (1) Human papillomavirus (1) Human thyroid cancer (1) Leukemia (1) P53 gene (1) Single-stranded conformation polymorphism (1) Suppressor gene (1) TRAP assay (1) Telomerase (1) Telomeric repeat amplification protocol (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Info:eu-repo/semantics/article (1) Ano 2002 (1) 2001 (1) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Detection of point mutations by non-isotopic single strand conformation polymorphism BJMBR Pinheiro,N.A.; Moura,R.P.; Monteiro,E.; Villa,L.L.. We have developed a procedure for nonradioactive single strand conformation polymorphism analysis and applied it to the detection of point mutations in the human tumor suppressor gene p53. The protocol does not require any particular facilities or equipment, such as radioactive handling, large gel units for sequencing, or a semiautomated electrophoresis system. This technique consists of amplification of DNA fragments by PCR with specific oligonucleotide primers, denaturation, and electrophoresis on small neutral polyacrylamide gels, followed by silver staining. The sensitivity of this procedure is comparable to other described techniques and the method is easy to perform and applicable to a variety of tissue specimens. Tipo: Info:eu-repo/semantics/other Palavras-chave: P53 gene; Human papillomavirus; PCR; SSCP; Cancer; Silver staining. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100008 Low frequency of p53 mutations in cervical carcinomas among Brazilian women BJMBR Pinheiro,N.A.; Villa,L.L.. Human papillomavirus (HPV) infections of the high-risk types are strongly linked to the development of cervical carcinoma. The HPV oncoproteins E6 and E7 are thought to play a crucial role in this process through their interactions with the p53 protein and the retinoblastoma susceptibility gene product pRb, respectively. E6 binds to p53 protein promoting its degradation. This is considered to contribute to the oncogenesis of HPV-associated anogenital cancer. On the other hand, in HPV-negative cervical carcinoma, p53 mutations are thought to have a role in the transformation process. A total of 122 HPV-positive cervical carcinoma tissue samples were evaluated for the presence of mutations in exons 5-8 of the p53 gene by single-stranded conformation... Tipo: Info:eu-repo/semantics/article Palavras-chave: Single-stranded conformation polymorphism; SSCP; Suppressor gene; HPV; PCR; DNA sequencing. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000600005 TRAP-silver staining, a highly sensitive assay for measuring telomerase activity in tumor tissue and cell lines BJMBR Dalla Torre,C.A.; Maciel,R.M.B.; Pinheiro,N.A.; Andrade,J.A.D.; de Toledo,S.R.C.; Villa,L.L.; Cerutti,J.M.. Measurement of telomerase activity in clinically obtained tumor samples may provide important information for use as both a diagnostic marker and a prognostic indicator for patient outcome. In order to evaluate telomerase activity in tumor tissue without radiolabeling the product, we developed a simple telomeric repeat amplification protocol-silver-staining assay that is less time-consuming, is safe and requires minimal equipment. In addition, we determined the sensitivity of the silver-staining method by using extracts of telomerase-positive thyroid carcinoma cell lines which were serially diluted from 5,000 to 10 cells. Telomerase activity was also assayed in 19 thyroid tumors, 2 normal controls and 27 bone marrow aspirates. The results indicate that the... Tipo: Info:eu-repo/semantics/other Palavras-chave: Telomeric repeat amplification protocol; TRAP assay; Human thyroid cancer; Leukemia; Telomerase; Silver staining. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000100009 Registros recuperados: 3 Primeira ... 1 ... Última

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